In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
Answer
The flow chart shows the three steps involved in the process of PCR showing the following -
- Denaturation The DNA strands are treated with a temperature of 940C (Heat) and the strands are separated.
- Annealing The primers anneal to the complementary strands
- Extension The DNA polymerase facilitates the extension of the strands.